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KMID : 0365819740140020181
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Journal of Pusan Medical College
1974 Volume.14 No. 2 p.181 ~ p.187
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The Effect of Carcinogenics on Oxidative Phosphorylation in Rat Liver Mitochondria
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Abstract
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Studies were performed to observe the effects of various carcinogenics such as 2-aminobiphenyl, benzoa-pyrene, 3, 3´-diaminobenzidine, j9-naphthylamine and p-dimethylaminoazobenzene on the oxidative phosphorylation of rat liver mitochondria.
For the in vivo experiment, rats were killed by decapitation 6 weeks after a subcutaneous injection of the carcinogenics, and the isolated liver mitochondria were incubated with standard incubation media.
The ATP formation and 02 uptake were measured.
In the ram of the in vitro experiment, the carcinogenics were added to incubation media containing liver mitochondria isolated from normal rat.
Results were summarized as follows.
(1) In vivo experiment.
1. 2-Aminobiphenyl:
The ATP formation and 02 uptake decreased as compared with control group using succinate as substrate.
No noticeable changes in ATP formation and 02 uptake were observed with glutamate as substrate.
2. Benzo-a-pyrene:
02 uptake decreased when succinate was used as substrate, but it increased if glutamate was the substrate.
3. 3, 3´-Diaminobenzidine:
With succinate as substrate, the ATP formation and 02 uptake decreased. The ATP formation and P:O value decreased with glutamate as substrate.
4. G-Naphthylamine:
The 02 uptake decreased, but P=0 value increased when succinate was used as substrate.
Using glutamate as substrate, ATP formation, 02 uptake and P:O value decreased.
5. p-Dimethylaminoazobenzene:
No noticeable changes in ATP formation, 0, uptake and P:O ratio were observed employing both succinate and glutamate as substrate.
(2) In vitro experiment.
The ATP formation and P:0 value were depressed by addition of benzo-a-pyrene, 3, 3´-diaminobenzidine and 13-naphthylamine to the incubation media containing mitochondria.
Howerver, 02 uptake was enhanced by them as compared with control.
The ATP formation and 0E uptake were decreased by 2-aminohiphenyl,but P:0 value was not affected.
p-Dimethylaminoazobenzene decreased OZ uptake, but it increased P:0 value.
The ATP formation was not changed in its value.
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